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Product Name
HER2/ErbB2 antibody
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Description
HER2/ErbB2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human kidney tissue, human lung tissue, human spleen tissue, human testis tissue. Positive FC detected in MCF-7 cells. Positive WB detected in HeLa cells, SGC-7901 cells. Positive IP detected in SGC-7901 cells. Observed molecular weight by Western-blot: 185 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CD340 antibody; ERBB2 antibody; HER 2 antibody; HER 2/neu antibody; HER2 antibody; MLN 19 antibody; MLN19 antibody; NEU antibody; NGL antibody; p185 antibody; p185erbB2 antibody; Proto oncogene c ErbB 2 antibody; Proto oncogene Neu antibody; TKR1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004448). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111295(ERBB2,p185-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111295(ERBB2,p185-Specific antibody) at dilution of 1:50 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug ERBB2,p185-Specific antibody (Catalog No:111295, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111295(ERBB2,p185-Specific antibody) at dilution of 1:2000
IP Result of anti-ERBB2,p185-Specific (IP:Catalog No:111295, 5ug; Detection:Catalog No:111295 1:500) with SGC-7901 cells lysate 2400ug.
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Background
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase- mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.
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References
- Contino F, Mazzarella C, Ferro A. Negative transcriptional control of ERBB2 gene by MBP-1 and HDAC1: diagnostic implications in breast cancer. BMC cancer. 13:81. 2013.
- Skelton LA, Boron WF. Effect of acute acid-base disturbances on ErbB1/2 tyrosine phosphorylation in rabbit renal proximal tubules. American journal of physiology. Renal physiology. 305(12):F1747-64. 2013.
- Maranto C, Perconti G, Contino F, Rubino P, Feo S, Giallongo A. Cellular stress induces cap-independent alpha-enolase/MBP-1 translation. FEBS letters. 589(16):2110-6. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"