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Hemoglobin epsilon antibody

Cat.#: 111288

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Product Information

  • Product Name
    Hemoglobin epsilon antibody
  • Documents
  • Description
    Hemoglobin epsilon Rabbit Polyclonal antibody. Positive FC detected in K-562 cells. Positive IHC detected in human placenta tissue. Positive WB detected in human placenta tissue, K-562 cells, mouse placenta tissue. Observed molecular weight by Western-blot: 12-16 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Epsilon globin antibody; HBE antibody; HBE1 antibody; Hemoglobin epsilon chain antibody; Hemoglobin subunit epsilon antibody; hemoglobin antibody; epsilon 1 antibody
  • Immunogen
    Human HBE1 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Hemoglobin epsilon recombinant protein (Accession Number: NM_005330). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111288(HBE1 antibody) at dilution of 1:1000

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111288(HBE1 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:111288(HBE1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:111288(HBE1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:111288(HBE1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:111288(HBE1 antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 K-562 cells were stained with 0.2ug HBE1 antibody (Catalog No:111288, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 K-562 cells were stained with 0.2ug HBE1 antibody (Catalog No:111288, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).
  • References
    • Wei L, Jia L, Zhu L. A comparison of E15.5 fetus and newborn rat serum proteomes. Proteome science. 10(1):64. 2012.
    • Ma Y, Wang B, Jiang F. A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression. Molecular and cellular biology. 33(20):3994-4007. 2013.
    • Starnes LM, Sorrentino A, Pelosi E. NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression. Blood. 114(9):1753-63. 2009.
    • Vian L, Di Carlo M, Pelosi E. Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors. Cell death and differentiation. 21(2):290-301. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"