Cart 2

HELLS antibody

Cat.#: 111400

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    HELLS antibody
  • Documents
  • Description
    HELLS Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse testis tissue. Positive WB detected in K-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells. Observed molecular weight by Western-blot: 97kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ10339 antibody; helicase antibody; lymphoid antibody; HELLS antibody; LSH antibody; Lymphoid specific helicase antibody; Nbla10143 antibody; PASG antibody; SMARCA6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HELLS recombinant protein (Accession Number: BC031004). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111400(HELLS antibody) at dilution of 1:500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111400(HELLS antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111400(HELLS antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111400(HELLS antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using HELLS antibody Catalog No:111400 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using HELLS antibody Catalog No:111400 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-HELLS (IP:Catalog No:111400, 4ug; Detection:Catalog No:111400 1:1000) with mouse testis tissue lysate 4000ug.

    IP Result of anti-HELLS (IP:Catalog No:111400, 4ug; Detection:Catalog No:111400 1:1000) with mouse testis tissue lysate 4000ug.

  • Background
    Lymphoid-specific helicase(HELLS) ia a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.
  • References
    • Dunican DS, Cruickshanks HA, Suzuki M. Lsh regulates LTR retrotransposon repression independently of Dnmt3b function. Genome biology. 14(12):R146. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"