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HDAC8 antibody

Cat.#: 111383

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Product Information

  • Product Name
    HDAC8 antibody
  • Documents
  • Description
    HDAC8 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in HeLa cells, Raji cells. Observed molecular weight by Western-blot: 49kd
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HD8 antibody; HDAC8 antibody; HDACL1 antibody; histone deacetylase 8 antibody; RPD3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HDAC8 recombinant protein (Accession Number: NM_018486). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111383(HDAC8 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111383(HDAC8 antibody) at dilution of 1:500

    IP Result of anti-HDAC8 (IP:Catalog No:111383, 4ug; Detection:Catalog No:111383 1:600) with Raji cells lysate 2800ug.

    IP Result of anti-HDAC8 (IP:Catalog No:111383, 4ug; Detection:Catalog No:111383 1:600) with Raji cells lysate 2800ug.

  • Background
    Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.
  • References
    • Shu L, Khor TO, Lee JH. Epigenetic CpG demethylation of the promoter and reactivation of the expression of Neurog1 by curcumin in prostate LNCaP cells. The AAPS journal. 13(4):606-14. 2011.
    • Wu TY, Khor TO, Su ZY. Epigenetic modifications of Nrf2 by 3,3'-diindolylmethane in vitro in TRAMP C1 cell line and in vivo TRAMP prostate tumors. The AAPS journal. 15(3):864-74. 2013.
    • Shen Q, Yao Q, Sun J. Downregulation of histone deacetylase 1 by microRNA-520h contributes to the chemotherapeutic effect of doxorubicin. FEBS letters. 588(1):184-91. 2014.
    • Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN. Apigenin reactivates Nrf2 anti-oxidative stress signaling in mouse skin epidermal JB6 P + cells through epigenetics modifications. The AAPS journal. 16(4):727-35. 2014.
    • Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014.
    • Guo Y, Shu L, Zhang C, Su ZY, Kong AN. Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochemical pharmacology. 94(2):69-78. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"