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HDAC5-specific antibody

Cat.#: 106978

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Product Information

  • Product Name
    HDAC5-specific antibody
  • Documents
  • Description
    HDAC5-specific Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human heart tissue, mouse brain tissue. Positive WB detected in human brain tissue, HEK-293 cells, HeLa cells, MCF-7 cells, rat brain tissue. Observed molecular weight by Western-blot: 124kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    Antigen NY CO 9 antibody; FLJ90614 antibody; HD5 antibody; HDAC5 antibody; histone deacetylase 5 antibody; KIAA0600 antibody; NY CO 9 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: XM_005256906). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111379(HDAC5-specific antibody) at dilution of 1:300

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111379(HDAC5-specific antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111379(HDAC5-specific antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111379(HDAC5-specific antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111379(HDAC5-specific antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111379(HDAC5-specific antibody) at dilution of 1:50 (under 40x lens)

  • Background
    Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.
  • References
    • He M, Zhang B, Wei X. HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury. Journal of cellular and molecular medicine. 17(4):531-42. 2013.
    • Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014.
    • Fang J, Li Y, Zhou K, Hua Y, Wang C, Mu D. Antithetical regulation of α-myosin heavy chain between fetal and adult heart failure though shuttling of HDAC5 regulating YY-1 function. Cardiovascular toxicology. 15(2):147-56. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"