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Product Name
HDAC4 antibody
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Description
HDAC4 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells. Positive IP detected in HeLa cells. Positive IHC detected in human kidney tissue, human cervical cancer tissue. Observed molecular weight by Western-blot: 119-140kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HA6116 antibody; HD4 antibody; HDAC A antibody; HDAC4 antibody; HDACA antibody; histone deacetylase 4 antibody; KIAA0288 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HDAC4 recombinant protein (Accession Number: BC039904). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111377(HDAC4 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111377(HDAC4 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-HDAC4 (IP:Catalog No:111377, 4ug; Detection:Catalog No:111377 1:500) with HeLa cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:111377(HDAC4 Antibody) at dilution of 1:50 (under 40x lens)
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Background
HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4.
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References
- Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014.
- Zhang X, Zuo X, Yang B. MicroRNA directly enhances mitochondrial translation during muscle differentiation. Cell. 158(3):607-19. 2014.
- Barbier-Torres L, Beraza N, Fernández-Tussy P. Histone deacetylase 4 promotes cholestatic liver injury in the absence of prohibitin-1. Hepatology (Baltimore, Md.). 62(4):1237-48. 2015.
- He M, Zhang B, Wei X. HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury. Journal of cellular and molecular medicine. 17(4):531-42. 2013.
- Cai M, Hu Z, Liu J. Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis. Oncology reports. 33(2):685-92. 2015.
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