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Product Name
HDAC3 antibody
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Description
HDAC3 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in HL-60 cells, A431 cells, HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HD3 antibody; HDAC3 antibody; histone deacetylase 3 antibody; RPD3 antibody; RPD3 2 antibody; SMAP45 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HDAC3 recombinant protein (Accession Number: NM_003883). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:111375(HDAC3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:111375(HDAC3 antibody) at dilution of 1:50 (under 40x lens)
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:111375(HDAC3 Antibody) at dilution of 1:1000
IP Result of anti-HDAC3 (IP:Catalog No:111375, 3ug; Detection:Catalog No:111375 1:300) with A431 cells lysate 1200ug.
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Background
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC3.
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References
- He M, Zhang B, Wei X. HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury. Journal of cellular and molecular medicine. 17(4):531-42. 2013.
- Hu X, Lu X, Liu R. Histone cross-talk connects protein phosphatase 1α (PP1α) and histone deacetylase (HDAC) pathways to regulate the functional transition of bromodomain-containing 4 (BRD4) for inducible gene expression. The Journal of biological chemistry. 289(33):23154-67. 2014.
- Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014.
- Yuan H, Niu Y, Liu X, Fu L. Exercise increases the binding of MEF2A to the Cpt1b promoter in mouse skeletal muscle. Acta physiologica (Oxford, England). 212(4):283-92. 2014.
- Seo J, Min SK, Park HR. Expression of Histone Deacetylases HDAC1, HDAC2, HDAC3, and HDAC6 in Invasive Ductal Carcinomas of the Breast. Journal of breast cancer. 17(4):323-31. 2014.
- Liu RZ, Garcia E, Glubrecht DD, Poon HY, Mackey JR, Godbout R. CRABP1 is associated with a poor prognosis in breast cancer: adding to the complexity of breast cancer cell response to retinoic acid. Molecular cancer. 14:129. 2015.
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