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HDAC1 antibody

Cat.#: 111370

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Product Information

  • Product Name
    HDAC1 antibody
  • Documents
  • Description
    HDAC1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, mouse testis tissue, rat testis tissue. Positive IP detected in mouse testis tissue. Positive FC detected in HeLa cells, SH-SY5Y cells. Positive IHC detected in human pancreas cancer tissue, human pancreas tissue, human testis tissue. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 60 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DKFZp686H12203 antibody; GON 10 antibody; HD1 antibody; HDAC1 antibody; histone deacetylase 1 antibody; RPD3 antibody; RPD3L1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HDAC1 recombinant protein (Accession Number: NM_004964). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111370(HDAC1 antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111370(HDAC1 antibody) at dilution of 1:500

    IP Result of anti-HDAC1 (IP:Catalog No:111370, 3ug; Detection:Catalog No:111370 1:500) with mouse testis tissue lysate 10000ug.

    IP Result of anti-HDAC1 (IP:Catalog No:111370, 3ug; Detection:Catalog No:111370 1:500) with mouse testis tissue lysate 10000ug.

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111370(HDAC1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111370(HDAC1 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of SH-SY5Y cells, using HDAC1 antibody Catalog No:111370 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).

    Immunofluorescent analysis of SH-SY5Y cells, using HDAC1 antibody Catalog No:111370 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).

    1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (Catalog No:111370, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (Catalog No:111370, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC1.
  • References
    • Ro H, Dawid IB. Modulation of Tcf3 repressor complex composition regulates cdx4 expression in zebrafish. The EMBO journal. 30(14):2894-907. 2011.
    • Chen S, Evans HG, Evans DR. FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short. PloS one. 7(3):e32971. 2012.
    • Liu Y, Zhang JB, Qin Y. PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability. Hepatology (Baltimore, Md.). 58(2):692-705. 2013.
    • Zhang ZH, Hao CL, Liu P. Valproic acid inhibits tumor angiogenesis in mice transplanted with Kasumi‑1 leukemia cells. Molecular medicine reports. 9(2):443-9. 2014.
    • Hu X, Lu X, Liu R. Histone cross-talk connects protein phosphatase 1α (PP1α) and histone deacetylase (HDAC) pathways to regulate the functional transition of bromodomain-containing 4 (BRD4) for inducible gene expression. The Journal of biological chemistry. 289(33):23154-67. 2014.
    • Shang J, Wan Q, Wang X. Identification of NOD2 as a novel target of RNA-binding protein HuR: evidence from NADPH oxidase-mediated HuR signaling in diabetic nephropathy. Free radical biology & medicine. 79:217-27. 2015.
    • Cai M, Hu Z, Liu J. Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis. Oncology reports. 33(2):685-92. 2015.
    • Shi Y, Xu LZ, Peng K. Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. Scientific reports. 5:17651. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"