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Product Name
HCFC1 antibody
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Description
HCFC1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human kidney tissue. Positive IF detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, Jurkat cells. Observed molecular weight by Western-blot: 230 kDa, 300 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C1 factor antibody; CFF antibody; HCF antibody; HCF 1 antibody; HCF1 antibody; HCFC1 antibody; HFC1 antibody; Host cell factor 1 antibody; VCAF antibody; VP16 accessory protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HCFC1 recombinant protein (Accession Number: BC063435). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111279(HCFC1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111279(HCFC1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111279(HCFC1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:111279(HCFC1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HCFC1 represents host cell factor C1 (VP16-accessory protein)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"