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Product Name
HARS2 antibody
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Description
HARS2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human kidney tissue. Positive WB detected in MCF7 cells, HEK-293 cells, human heart tissue, mouse kidney tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HARS2 antibody; HARSL antibody; HARSR antibody; HisRS antibody; Histidine tRNA ligase antibody; Histidine tRNA ligase like antibody; HO3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HARS2 recombinant protein (Accession Number: NM_012208). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111257(HARS2 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111257(HARS2 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-HARS2 (IP:Catalog No:111257, 4ug; Detection:Catalog No:111257 1:500) with HEK-293 cells lysate 3000ug.
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Background
HARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"