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Product Name
HACL1 antibody
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Description
HACL1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue, human skeletal muscle tissue, rat kidney tissue, rat liver tissue. Observed molecular weight by Western-blot: 64 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
2 HPCL antibody; 2 hydroxyacyl CoA lyase 1 antibody; 2 hydroxyphytanoyl CoA lyase antibody; FLJ55041 antibody; HACL1 antibody; HPCL antibody; HPCL2 antibody; PHYH2 antibody; Phytanoyl CoA 2 hydroxylase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HACL1 recombinant protein (Accession Number: NM_012260). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111331(HACL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:111331(HACL1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111331(HACL1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:111331(HACL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HACL1 represents 2-hydroxyacyl-CoA lyase 1
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