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Product Name
GSR antibody
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Description
GSR Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, human placenta tissue, mouse lung tissue. Positive IP detected in HeLa cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 52-55 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GLUR antibody; glutathione reductase antibody; GRase antibody; GRD1 antibody; GSR antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GSR recombinant protein (Accession Number: NM_000637). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111177(GSR antibody) at dilution of 1:500
Immunofluorescent analysis of MCF-7 cells, using GSR antibody Catalog No:111177 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-GSR (IP:Catalog No:111177, 4ug; Detection:Catalog No:111177 1:500) with HeLa cells lysate 2600ug.
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Background
GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.
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References
- Wu H, Xiao Y, Zhang S. The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Cell reports. 3(5):1663-77. 2013.
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