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Product Name
GSNOR,ADH5 antibody
- Documents
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Description
GSNOR,ADH5 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human liver tissue, human endometrial cancer tissue, human pancreas cancer tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, human brain tissue, human liver tissue, human testis tissue, mouse brain tissue, mouse heart tissue. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, IHC, IP, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADH 3 antibody; ADH5 antibody; ADHX antibody; Alcohol dehydrogenase 5 antibody; Alcohol dehydrogenase class 3 antibody; FALDH antibody; FDH antibody; GSH FDH antibody; GSNOR antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GSNOR,ADH5 recombinant protein (Accession Number: NM_000671). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111173(GSNOR,ADH5 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human liver using Catalog No:111173(GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111173(GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-GSNOR,ADH5 (IP:Catalog No:111173, 3ug; Detection:Catalog No:111173 1:800) with mouse liver tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:111173(GSNOR,ADH5 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug GSNOR,ADH5 antibody (Catalog No:111173, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"