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Product Name
GRIPAP1 antibody
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Description
GRIPAP1 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human brain tissue. Positive WB detected in human brain tissue, HeLa cells. Observed molecular weight by Western-blot: 97kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434P0630 antibody; GRASP 1 antibody; GRIP1 assOCiated protein 1 antibody; GRIPAP1 antibody; KIAA1167 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GRIPAP1 recombinant protein (Accession Number: BC001522). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111210(GRIPAP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:111210(GRIPAP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:111210(GRIPAP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:111210(GRIPAP1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GRIP-associated protein-1 (GRASP-1), also named as GRIPAP1, is a neuron-specific effector of the small GTPase Rab4 and key component of AMPA receptor recycling machinery in dendrites. GRASP-1 is essential for maintenance of spine morphology and important for LTP. GRASP-1 connects Rab4 and Rab11 recycling endosomal domains through the interaction with target (t)-SNARE syntaxin. The MW of this protein is 97 kDa, and Catalog#15806-1-AP specially recognises the 97 kDa protein.
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