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Product Name
GRIM19 antibody
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Description
GRIM19 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in human placenta tissue, HEK-293 cells, HeLa cells, Jurkat cells, MCF7 cells. Positive IHC detected in human prostate cancer tissue, human heart tissue, human kidney tissue. Positive IF detected in MCF-7 cells, HeLa cells. Observed molecular weight by Western-blot: 16.5kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B16.6 antibody; CDA016 antibody; CGI 39 antibody; CI B16.6 antibody; Complex I B16.6 antibody; GRIM 19 antibody; GRIM19 antibody; NDUFA13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GRIM19 recombinant protein (Accession Number: NM_015965). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111208(GRIM19 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111208(GRIM19 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-GRIM19 (IP:Catalog No:111208, 4ug; Detection:Catalog No:111208 1:500) with HEK-293 cells lysate 2000ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:111208(GRIM19 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"