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Product Name
GPX4 antibody
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Description
GPX4 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse kidney tissue, human testis tissue, mouse testis tissue. Positive IHC detected in human kidney tissue, human testis tissue. Observed molecular weight by Western-blot: 20kd-23kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Glutathione peroxidase 4 antibody; GPx 4 antibody; GPX4 antibody; GSHPx 4 antibody; MCSP antibody; PHGPx antibody; snGPx antibody; snPHGPx antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GPX4 recombinant protein (Accession Number: NM_002085). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:111149(GPX4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111149(GPX4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111149(GPX4 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-GPX4 (IP:Catalog No:111149, 4ug; Detection:Catalog No:111149 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers(PMID:17630701).It presents primarily in testis.
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References
- Chen X, Xu W, Miao M. Alteration of sperm protein profile induced by cigarette smoking. Acta biochimica et biophysica Sinica. 47(7):504-15. 2015.
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