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GPRC5A,RAI3 antibody

Cat.#: 111139

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Product Information

  • Product Name
    GPRC5A,RAI3 antibody
  • Documents
  • Description
    GPRC5A,RAI3 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IHC detected in human lung cancer tissue, human breast cancer tissue. Positive FC detected in MCF-7 cells.
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    GPCR5A antibody; GPRC5A antibody; RAI3 antibody; RAIG 1 antibody; RAIG1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GPRC5A,RAI3 recombinant protein (Accession Number: NM_003979). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:50-1:500

  • Validations

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111139(GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111139(GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111139(GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111139(GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111139(GPRC5A,RAI3 Antibody) at dilution of 1:700

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111139(GPRC5A,RAI3 Antibody) at dilution of 1:700

    1X10^6 MCF-7 cells were stained with 0.2ug GPRC5A,RAI3 antibody (Catalog No:111139, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug GPRC5A,RAI3 antibody (Catalog No:111139, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"