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GPI antibody

Cat.#: 111064

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Product Information

  • Product Name
    GPI antibody
  • Documents
  • Description
    GPI Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 63 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AMF antibody; AutOCrine motility factor antibody; g6pi antibody; Glucose 6 phosphate isomerase antibody; glucose phosphate isomerase antibody; GNPI antibody; GPI antibody; Neuroleukin antibody; NLK antibody; PGI antibody; PHI antibody; Phosphoglucose isomerase antibody; Phosphohexose isomerase antibody; SA 36 antibody; Sperm antigen 36 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GPI recombinant protein (Accession Number: NM_000175). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111064(GPI antibody) at dilution of 1:500

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111064(GPI antibody) at dilution of 1:500

    IP Result of anti-GPI (IP:Catalog No:111064, 4ug; Detection:Catalog No:111064 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-GPI (IP:Catalog No:111064, 4ug; Detection:Catalog No:111064 1:500) with HeLa cells lysate 1200ug.

  • Background
    GPI(Glucose-6-phosphate isomerase),which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI), sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis(PMID:12783864). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form(PMID:11371164). It has 2 isoforms produced by alternative splicing.
  • References
    • Lu Y, Cai G, Cui S. FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell. Proteomics. 14(21-22):2485-97. 2014.
    • Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"