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Product Name
GORASP2 antibody
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Description
GORASP2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, rat testis tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 59 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434D156 antibody; FLJ13139 antibody; Golgi phosphoprotein 6 antibody; GOLPH6 antibody; GORASP2 antibody; GRASP55 antibody; GRS2 antibody; p59 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GORASP2 recombinant protein (Accession Number: NM_015530). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111048(GORASP2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111048(GORASP2 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using GORASP2 antibody Catalog No:111048 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).
IP Result of anti-GORASP2 (IP:Catalog No:111048, 3ug; Detection:Catalog No:111048 1:700) with HeLa cells lysate 1320ug.
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Background
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
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References
- Dupont N, Jiang S, Pilli M, Ornatowski W, Bhattacharya D, Deretic V. Autophagy-based unconventional secretory pathway for extracellular delivery of IL-1β. The EMBO journal. 30(23):4701-11. 2011.
- Xiang Y, Zhang X, Nix DB. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nature communications. 4:1659. 2013.
- Zhang X, Zhang H, Wang Y. Phosphorylation regulates VCIP135 function in Golgi membrane fusion during the cell cycle. Journal of cell science. 127(Pt 1):172-81. 2014.
- Tian G, Ropelewski P, Nemet I, Lee R, Lodowski KH, Imanishi Y. An unconventional secretory pathway mediates the cilia targeting of peripherin/rds. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34(3):992-1006. 2014.
- Lee I, Tiwari N, Dunlop MH, Graham M, Liu X, Rothman JE. Membrane adhesion dictates Golgi stacking and cisternal morphology. Proceedings of the National Academy of Sciences of the United States of America. 111(5):1849-54. 2014.
- Ferraro F, Kriston-Vizi J, Metcalf DJ. A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells. Developmental cell. 29(3):292-304. 2014.
- Lavieu G, Dunlop MH, Lerich A, Zheng H, Bottanelli F, Rothman JE. The Golgi ribbon structure facilitates anterograde transport of large cargoes. Molecular biology of the cell. 25(19):3028-36. 2014.
- Xiang Y, Wang Y. GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking. The Journal of cell biology. 188(2):237-51. 2010.
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