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Product Name
GNE antibody
- Documents
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Description
GNE Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human placenta tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 79 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DMRV antibody; GLCNE antibody; GNE antibody; IBM2 antibody; ManAc kinase antibody; Uae1 antibody; UDP GlcNAc 2 epimerase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNE recombinant protein (Accession Number: NM_005476). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111000(GNE Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111000(GNE Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111000(GNE Antibody) at dilution of 1:50 (under 40x lens)
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Background
The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"