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GNB5 antibody

Cat.#: 110999

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Product Information

  • Product Name
    GNB5 antibody
  • Documents
  • Description
    GNB5 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue. Positive IP detected in rat brain tissue. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 39-42 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ37457 antibody; FLJ43714 antibody; GB5 antibody; Gbeta5 antibody; GNB5 antibody; Transducin beta chain 5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GNB5 recombinant protein (Accession Number: BC011671). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110999(GNB5 antibody) at dilution of 1:1000

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110999(GNB5 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110999(GNB5 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110999(GNB5 antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-GNB5 (IP:Catalog No:110999, 4ug; Detection:Catalog No:110999 1:500) with rat brain tissue lysate 4000ug.

    IP Result of anti-GNB5 (IP:Catalog No:110999, 4ug; Detection:Catalog No:110999 1:500) with rat brain tissue lysate 4000ug.

  • Background
    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.
  • References
    • Fuchs D, Metzig M, Bickeböller M, Brandel C, Roth W. The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma. Oncogene. 34(21):2753-63. 2015.
    • Tracy CM, Kolesnikov AV, Blake DR. Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling. PloS one. 10(2):e0117129. 2015.
    • Saba LM, Bennett B, Hoffman PL. A systems genetic analysis of alcohol drinking by mice, rats and men: influence of brain GABAergic transmission. Neuropharmacology. 60(7-8):1269-80. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"