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Product Name
GNB2 antibody
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Description
GNB2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells. Observed molecular weight by Western-blot: 37kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
G protein subunit beta 2 antibody; GNB2 antibody; Transducin beta chain 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNB2 recombinant protein (Accession Number: NM_005273). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110995(GNB2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110995(GNB2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110995(GNB2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GNB2 antibody Catalog No:110995 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
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