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Product Name
GNAS antibody
- Documents
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Description
GNAS Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue. Positive IHC detected in human pancreas tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, IP, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AHO antibody; C20orf45 antibody; dJ309F20.1.1 antibody; dJ806M20.3.3 antibody; GNAS antibody; GNAS complex lOCus antibody; GNAS1 antibody; GPSA antibody; GSA antibody; GSP antibody; NESP antibody; PHP1A antibody; PHP1B antibody; POH antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNAS recombinant protein (Accession Number: NM_000516). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110992(NESP55,GNAS Antibody) at dilution of 1:1000
IP Result of anti-NESP55,GNAS (IP:Catalog No:110992, 4ug; Detection:Catalog No:110992 1:300) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:110992(NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:110992(NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens)
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Background
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
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References
- Gemoll T, Roblick UJ, Szymczak S. HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers. Cellular and molecular life sciences : CMLS. 68(19):3261-74. 2011.
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