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Product Name
GNAO1 antibody
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Description
GNAO1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in rat brain tissue, human brain tissue, human liver tissue, mouse testis tissue, Y79 cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686O0962 antibody; G ALPHA o antibody; GNAO antibody; GNAO1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNAO1 recombinant protein (Accession Number: BC030027). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110990(GNAO1 Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110990(GNAO1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-GNAO1 (IP:Catalog No:110990, 3ug; Detection:Catalog No:110990 1:600) with mouse brain tissue lysate 3600ug.
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Background
GNAO1 represents guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
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References
- Pei X, Zhang J, Wu L. The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma. Bioscience reports. 33(5):. 2013.
- Liu Z, Zhang J, Wu L, Liu J, Zhang M. Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis. International journal of molecular medicine. 33(3):589-96. 2014.
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