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Product Name
GNAI3 antibody
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Description
GNAI3 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human brain tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse kidney tissue, HEK-293 cells, human kidney tissue, mouse brain tissue, mouse heart tissue. Observed molecular weight by Western-blot: 41-45kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
87U6 antibody; FLJ26559 antibody; G(i) alpha 3 antibody; GNAI3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNAI3 recombinant protein (Accession Number: NM_006496). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:110989(GNAI3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110989(GNAI3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110989(GNAI3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GNAI3 antibody Catalog No:110989 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
GNAI3 represents guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
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