-
Product Name
GMIP antibody
- Documents
-
Description
GMIP Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 130 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
GEM interacting protein antibody; GMIP antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of GMIP recombinant protein (Accession Number: NM_016573). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111100(GMIP Antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells using Catalog No:111100(GMIP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"