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Product Name
Glutamate receptor 2 antibody
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Description
Glutamate receptor 2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue, SKOV-3 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 94-105 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GluA2 antibody; GluR 2 antibody; GluR B antibody; GluR K2 antibody; GLUR2 antibody; GLURB antibody; Glutamate receptor 2 antibody; GRIA2 antibody; HBGR2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Glutamate receptor 2 recombinant protein (Accession Number: NM_001083619). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111080(GRIA2 antibody) at dilution of 1:1000
IP Result of anti-GRIA2 (IP:Catalog No:111080, 4ug; Detection:Catalog No:111080 1:500) with mouse brain tissue lysate 4000ug.
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Background
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.
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References
- Casimiro TM, Sossa KG, Uzunova G, Beattie JB, Marsden KC, Carroll RC. mGluR and NMDAR activation internalize distinct populations of AMPARs. Molecular and cellular neurosciences. 48(2):161-70. 2011.
- Casimiro TM, Nawy S, Carroll RC. Molecular mechanisms underlying activity-dependent AMPA receptor cycling in retinal ganglion cells. Molecular and cellular neurosciences. 56:384-92. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"