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Product Name
GLUT2 antibody
- Documents
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Description
GLUT2 Rabbit Polyclonal antibody. Positive WB detected in rat liver tissue, mouse kidney tissue, mouse small intestine tissue. Positive IP detected in mouse kidney tissue. Positive IHC detected in human small intestine tissue. Observed molecular weight by Western-blot: 60-70 kDa, 38-45 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Rat, Mouse; other species not tested.
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Alternative names
GLUT 2 antibody; GLUT2 antibody; SLC2A2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GLUT2 recombinant protein (Accession Number: XM_047448761). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:111077(SLC2A2 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:111077(SLC2A2 Antibody) at dilution of 1:50 (under 10x lens)
rat liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111077(SLC2A2 Antibody) at dilution of 1:300
IP Result of anti-SLC2A2 (IP:Catalog No:111077, 4ug; Detection:Catalog No:111077 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
GLUT2 is a member of glucose transporters or GLUTs which catalyze the glucose transport across plasma membrane. GLUT2 is selectively expressed in hepatocytes, pancreatic beta cells, and absorptive renal and intestinal epithelial cells. GLUT2 is required to maintain normal glucose homeostasis and normal function and development of the endocrine pancreas. Its absence leads to symptoms characteristic of non-insulin-dependent diabetes mellitus. This antibody detected 60-70 kDa fully glycosylated, matured GLUT2 and a nonglycosylated form of GLUT2 around 38-45 kDa. (9354799, 12101013)
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