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Product Name
Glucocorticoid receptor antibody
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Description
Glucocorticoid receptor Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, A431 cells, C2C12 cells, HepG2 cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 94-97 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
GCCR antibody; GCR antibody; GlucOCorticoid receptor antibody; GRL antibody; NR3C1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Gluc℃orticoid receptor recombinant protein (Accession Number: XM_017009397). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111041(NR3C1 antibody) at dilution of 1:1000
C2C12 cells (shcontrol and shRNA of NR3C1) were subjected to SDS PAGE followed by western blot with Catalog No:111041(NR3C1 antibody) at dilution of 1:1000. (Data provided by Angran Biotech (www.miRNAlab.com)).
Immunofluorescent analysis of HepG2 cells, using NR3C1 antibody Catalog No:111041 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-Glucocorticoid receptor (IP:Catalog No:111041, 4ug; Detection:Catalog No:111041 1:500) with mouse heart tissue lysate 4400ug.
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Background
NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058]. It also act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth [PMID:15037546]. This antibody is specific to NR3C1.
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References
- Matthews LC, Berry AA, Morgan DJ. Glucocorticoid receptor regulates accurate chromosome segregation and is associated with malignancy. Proceedings of the National Academy of Sciences of the United States of America. 112(17):5479-84. 2015.
- Hu YW, Ma X, Huang JL. Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet. PloS one. 8(6):e66876. 2013.
- Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW. Pin1 promotes GR transactivation by enhancing recruitment to target genes. Nucleic acids research. 41(18):8515-25. 2013.
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