-
Product Name
GLI2-Specific antibody
- Documents
-
Description
GLI2-Specific Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human testis tissue. Positive IF detected in Hela cells, MCF-7 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 88kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
AW546128 antibody; GLI Kruppel family member GLI2 antibody; Gli2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001081125). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111021(GLI2-Specific antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111021(GLI2-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111021(GLI2-Specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using Gli2 antibody Catalog No:111021 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9).
-
References
- Pang P, Shimo T, Takada H. Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction. Biochemical and biophysical research communications. 467(1):21-6. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"