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Product Name
GFM1 antibody
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Description
GFM1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in mouse kidney tissue, HeLa cells, human heart tissue. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COXPD1 antibody; EF Gmt antibody; EFG antibody; EFG1 antibody; EFGM antibody; EGF1 antibody; Elongation factor G1 antibody; FLJ12662 antibody; FLJ13632 antibody; FLJ20773 antibody; GFM antibody; GFM1 antibody; hEFG1 antibody; mEF G 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GFM1 recombinant protein (Accession Number: NM_024996). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:110947(GFM1 antibody) at dilution of 1:500
IP Result of anti-GFM1 (IP:Catalog No:110947, 4ug; Detection:Catalog No:110947 1:1000) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:110947(GFM1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]
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References
- Galmiche L, Serre V, Beinat M. Toward genotype phenotype correlations in GFM1 mutations. Mitochondrion. 12(2):242-7. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"