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Product Name
gephyrin antibody
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Description
gephyrin Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, Jurkat cells. Positive IP detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 93 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Domain E antibody; GEPH antibody; gephyrin antibody; GPH antibody; GPHN antibody; GPHRYN antibody; KIAA1385 antibody; MPT adenylyltransferase antibody; MPT Mo transferase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of gephyrin recombinant protein (Accession Number: NM_020806). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110943(GPHN antibody) at dilution of 1:400
IP Result of anti-GPHN (IP:Catalog No:110943, 4ug; Detection:Catalog No:110943 1:700) with HEK-293 cells lysate 3200ug.
Immunofluorescent analysis of HEK-293 cells using Catalog No:110943(GPHN Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.
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