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Product Name
GATA2 antibody
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Description
GATA2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells, mouse heart tissue, mouse liver tissue, Raji cells, rat heart tissue, rat liver tissue. Positive IHC detected in human lymphoma tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 60-66kd
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ45948 antibody; GATA binding protein 2 antibody; GATA2 antibody; NFE1B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GATA2 recombinant protein (Accession Number: NM_001145662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110879(GATA2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:110879(GATA2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using GATA2 antibody Catalog No:110879 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.
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References
- Ma Y, Yao N, Liu G. Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells. The EMBO journal. 34(3):361-78. 2015.
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