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Product Name
GAS7 antibody
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Description
GAS7 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, mouse testis tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 38-50 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GAS 7 antibody; GAS7 antibody; growth arrest 7 antibody; KIAA0394 antibody; MLL/GAS7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GAS7 recombinant protein (Accession Number: XM_005256832). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110873(GAS7 antibody) at dilution of 1:1000
IP Result of anti-GAS7 (IP:Catalog No:110873, 4ug; Detection:Catalog No:110873 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110873(GAS7 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Growth arrest-specific (GAS) genes are expressed preferentially in cells that enter a quiescent state. Gas7, first identified in serum-starved murine fibroblasts, encodes a 48-kDa protein. 3 isoforms of Gas7 were identified in rat brains, with molecular weight of 38, 47 and 55 kDa in SDS Page.
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References
- Ji Y, Shen M, Wang X. Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins. Journal of proteome research. 11(6):3186-98. 2012.
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