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Product Name
GARS antibody
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Description
GARS Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in human brain tissue, human liver tissue, human testis tissue. Positive IHC detected in human skin cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 75-80 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AP 4 A synthetase antibody; CMT2D antibody; DSMAV antibody; GARS antibody; Glycine tRNA ligase antibody; glycyl tRNA synthetase antibody; GlyRS antibody; HMN5 antibody; SMAD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GARS recombinant protein (Accession Number: NM_002047). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110867(GARS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110867(GARS antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110867(GARS antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GARS antibody Catalog No:110867 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GARS (IP:Catalog No:110867, 4ug; Detection:Catalog No:110867 1:500) with mouse liver tissue lysate 4000ug.
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Background
GARS(glycinamide ribonucleotide synthetase) perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA.GARS, however, is a ubiquitously expressed α2 homodimer responsible for charging tRNA molecules with glycine, this enzyme is essential for protein synthesis in all cells and tissues(PMID:17035524 ).It can exist as a oligomer with 250KDa(PMID:22504945).
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References
- Niehues S, Bussmann J, Steffes G. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nature communications. 6:7520. 2015.
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