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Product Name
GANP antibody
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Description
GANP Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 75-80 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
80 kDa MCM3 assOCiated protein antibody; FLJ44336 antibody; FLJ45306 antibody; GANP antibody; KIAA0572 antibody; MAP80 antibody; MCM3AP antibody; Protein GANP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GANP recombinant protein (Accession Number: BC013285). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:10-1:100
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110862(GANP antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110862(GANP antibody) at dilution of 1:10 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using MCM3AP antibody Catalog No:110862 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-GANP (IP:Catalog No:110862, 4ug; Detection:Catalog No:110862 1:500) with mouse brain tissue lysate 4000ug.
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Background
The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.
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References
- Singh SK, Maeda K, Eid MM. GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nature communications. 4:1830. 2013.
- Maeda K, Almofty SA, Singh SK. GANP interacts with APOBEC3G and facilitates its encapsidation into the virions to reduce HIV-1 infectivity. Journal of immunology (Baltimore, Md. : 1950). 191(12):6030-9. 2013.
- Maeda K, Singh SK, Eda K. GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA. The Journal of biological chemistry. 285(31):23945-53. 2010.
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