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Product Name
Galectin 4 antibody
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Description
Galectin 4 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in COLO 320 cells. Positive FC detected in MCF-7 cells. Positive WB detected in Jurkat cells, COLO 320 cells. Observed molecular weight by Western-blot: 38kd
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Antigen NY CO 27 antibody; Gal 4 antibody; GAL4 antibody; Galectin 4 antibody; L 36 lactose binding protein antibody; L36LBP antibody; Lactose binding lectin 4 antibody; LGALS4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Galectin 4 recombinant protein (Accession Number: NM_006149). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110837(LGALS4,GAL4 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110837(LGALS4,GAL4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110837(LGALS4,GAL4 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of COLO 320 cells using Catalog No:110837(LGALS4,GAL4 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug LGALS4,GAL4 antibody (Catalog No:110837, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
LGALS4 represents lectin, galactoside-binding, soluble, 4
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