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GABARAPL2-Specific antibody

Cat.#: 110802

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Product Information

  • Product Name
    GABARAPL2-Specific antibody
  • Documents
  • Description
    GABARAPL2-Specific Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human brain tissue. Positive FC detected in HepG2 cells. Positive WB detected in COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells. Observed molecular weight by Western-blot: 14 kDa
  • Tested applications
    ELISA, IHC, WB, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATG8 antibody; FLC3A antibody; GABARAPL2 antibody; GATE 16 antibody; GATE16 antibody; GEF 2 antibody; GEF2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_007285). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110802(GABARAPL2-Specific antibody) at dilution of 1:800

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110802(GABARAPL2-Specific antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human heart using Catalog No:110802(GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human heart using Catalog No:110802(GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human heart using Catalog No:110802(GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human heart using Catalog No:110802(GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 HepG2 cells were stained with 0.2ug GABARAPL2-Specific antibody (Catalog No:110802, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HepG2 cells were stained with 0.2ug GABARAPL2-Specific antibody (Catalog No:110802, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.
  • References
    • Tanji K, Odagiri S, Maruyama A. Alteration of autophagosomal proteins in the brain of multiple system atrophy. Neurobiology of disease. 49:190-8. 2013.
    • Fetalvero KM, Yu Y, Goetschkes M. Defective autophagy and mTORC1 signaling in myotubularin null mice. Molecular and cellular biology. 33(1):98-110. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"