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Product Name
GABARAPL1 antibody
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Description
GABARAPL1 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human kidney tissue. Positive WB detected in mouse kidney tissue, human heart tissue, human spleen tissue, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 17 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
APG8 LIKE antibody; APG8L antibody; ATG8 antibody; ATG8L antibody; GABARAPL1 antibody; GEC 1 antibody; GEC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_031412). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:110771(GABARAPL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110771(GABARAPL1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110771(GABARAPL1 antibody) at dilution of 1:100 (under 40x lens)
1X10^6 HepG2 cells were stained with 0.2ug GABARAPL1 antibody (Catalog No:110771, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It’s also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. And at very low levels in thymus and small intestine.The antibody is specific to GABARAPL1, and it doesn’t recognize the recombinant GABARAP and GABARAPL2.
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References
- Le Grand JN, Chakrama FZ, Seguin-Py S. GABARAPL1 antibodies: target one protein, get one free! Autophagy. 7(11):1302-7. 2011.
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