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Product Name
FUT4 antibody
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Description
FUT4 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue, human gliomas tissue, human skin cancer tissue. Positive WB detected in HeLa cells, HepG2 cells, HL-60 cells, Jurkat cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 95-110 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Alpha (1 antibody; 3) fucosyltransferase antibody; CD15 antibody; ELFT antibody; FCT3A antibody; FUC TIV antibody; Fucosyltransferase 4 antibody; Fucosyltransferase IV antibody; FucT IV antibody; FUT4 antibody; FUTIV antibody; SSEA 1 antibody; SSEA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002033). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110799(FUT4 antibody) at dilution of 1:500
IP Result of anti-FUT4 (IP:Catalog No:110799, 4ug; Detection:Catalog No:110799 1:500) with HeLa cells lysate 2480ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110799(FUT4 Antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110799(FUT4 Antibody) at dilution of 1:100 (under 40x lens)
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Background
FUT4,also named as CD15, SSEA-1ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. FUT4 is Stage-specific embryonic antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation.
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References
- Yang X, Liu S, Yan Q. Role of fucosyltransferase IV in epithelial-mesenchymal transition in breast cancer cells. Cell death & disease. 4:e735. 2013.
- Aziz F, Wang X, Liu J, Yan Q. Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions. Toxicology in vitro : an international journal published in association with BIBRA. 31:158-66. 2016.
- Tian L, Shen D, Li X. Ginsenoside Rg3 inhibits epithelial-mesenchymal transition (EMT) and invasion of lung cancer by down-regulating FUT4. Oncotarget. 7(2):1619-32. 2016.
- Aziz F, Yang X, Wang X, Yan Q. Anti-LeY antibody enhances therapeutic efficacy of celecoxib against gastric cancer by downregulation of MAPKs/COX-2 signaling pathway: correlation with clinical study. Journal of cancer research and clinical oncology. 141(7):1221-35. 2015.
- Shan X, Aziz F, Tian LL, Wang XQ, Yan Q, Liu JW. Ginsenoside Rg3-induced EGFR/MAPK pathway deactivation inhibits melanoma cell proliferation by decreasing FUT4/LeY expression. International journal of oncology. 46(4):1667-76. 2015.
- Zhang YM, Zhang YY, Bulbul A, Shan X, Wang XQ, Yan Q. Baicalin promotes embryo adhesion and implantation by upregulating fucosyltransferase IV (FUT4) via Wnt/beta-catenin signaling pathway. FEBS letters. 589(11):1225-33. 2015.
- Shan X, Tian LL, Zhang YM, Wang XQ, Yan Q, Liu JW. Ginsenoside Rg3 suppresses FUT4 expression through inhibiting NF-κB/p65 signaling pathway to promote melanoma cell death. International journal of oncology. 47(2):701-9. 2015.
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