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FUBP1 antibody

Cat.#: 110788

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Special Price 239.0 USD

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Product Information

  • Product Name
    FUBP1 antibody
  • Documents
  • Description
    FUBP1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in SH-SY5Y cells, Jurkat cells, K-562 cells, mouse brain tissue. Positive IP detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 69 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DNA helicase V antibody; FBP antibody; FUBP antibody; FUBP1 antibody; FUSE binding protein 1 antibody; hDH V antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FUBP1 recombinant protein (Accession Number: XM_017002743). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IF: 1:10-1:100

  • Validations

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:110788(FUBP1 Antibody) at dilution of 1:1000

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:110788(FUBP1 Antibody) at dilution of 1:1000

    IP Result of anti-FUBP1 (IP:Catalog No:110788, 4ug; Detection:Catalog No:110788 1:300) with SH-SY5Y cells lysate 800ug.

    IP Result of anti-FUBP1 (IP:Catalog No:110788, 4ug; Detection:Catalog No:110788 1:300) with SH-SY5Y cells lysate 800ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:110788(FUBP1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of HeLa cells using Catalog No:110788(FUBP1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

  • Background
    FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"