-
Product Name
FOXM1 antibody
- Documents
-
Description
FOXM1 Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, human testis tissue, L02 cells, mouse colon tissue, mouse spleen tissue, mouse testis tissue. Positive IP detected in L02 cells. Observed molecular weight by Western-blot: 110kd
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FKHL16 antibody; forkhead box M1 antibody; Forkhead box protein M1 antibody; FOXM1 antibody; FOXM1B antibody; HFH 11 antibody; HFH11 antibody; HNF 3 antibody; HNF 3/fork head homolog 11 antibody; INS 1 antibody; M phase phosphoprotein 2 antibody; MPHOSPH2 antibody; MPP 2 antibody; MPP2 antibody; PIG29 antibody; TGT3 antibody; Transcription factor Trident antibody; TRIDENT antibody; WIN antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of FOXM1 recombinant protein (Accession Number: NM_202003). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:110702(FOXM1 antibody) at dilution of 1:1000
IP Result of anti-FOXM1 (IP:Catalog No:110702, 5ug; Detection:Catalog No:110702 1:700) with L02 cells lysate 1560ug.
-
Background
Forkhead box M1 (FOXM1), also named HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1,FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa)
-
References
- Wen N, Wang Y, Wen L. Overexpression of FOXM1 predicts poor prognosis and promotes cancer cell proliferation, migration and invasion in epithelial ovarian cancer. Journal of translational medicine. 12:134. 2014.
- Chen X, Meng Q, Wang C. Protective effects of calycosin against CCl4-induced liver injury with activation of FXR and STAT3 in mice. Pharmaceutical research. 32(2):538-48. 2015.
- Zhang B, Liu LL, Mao X, Zhang DH. Effects of metformin on FOXM1 expression and on the biological behavior of acute leukemia cell lines. Molecular medicine reports. 10(6):3193-8. 2014.
- Zhang M, Liu Y, Gao Y, Li S. Silibinin-induced glioma cell apoptosis by PI3K-mediated but Akt-independent downregulation of FoxM1 expression. European journal of pharmacology. 765:346-54. 2015.
- Wang B, Ma A, Zhang L. POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. Nature communications. 6:8704. 2015.
- Xu Q, Fu R, Yin G, Liu X, Liu Y, Xiang M. Microarray-based gene expression profiling reveals genes and pathways involved in the oncogenic function of REG3A on pancreatic cancer cells. Gene. 578(2):263-73. 2016.
- Guan P, Chen H, Li HJ, Duan J, Chen JY. Expression and significance of FOXM1 in human cervical cancer: a tissue micro-array study. Clinical and investigative medicine. Médecine clinique et experimentale. 34(1):E1-7. 2011.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"