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Product Name
FOXA2 antibody
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Description
FOXA2 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue, human colon cancer tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, A549 cells, PC-13 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, IP, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
forkhead box A2 antibody; Forkhead box protein A2 antibody; FOXA2 antibody; HNF 3 beta antibody; HNF 3B antibody; HNF3B antibody; TCF 3B antibody; TCF3B antibody; Transcription factor 3B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FOXA2 recombinant protein (Accession Number: NM_153675). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110746(FOXA2 Antibody) at dilution of 1:1000
IP Result of anti-FOXA2 (IP:Catalog No:110746, 3ug; Detection:Catalog No:110746 1:300) with HepG2 cells lysate 1920ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110746(FOXA2 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:110746(FOXA2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:110746(FOXA2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.
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