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Product Name
FNDC5 antibody
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Description
FNDC5 Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue. Positive IP detected in mouse liver tissue. Positive WB detected in mouse placenta tissue, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 25kDa - 30 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FNDC5 antibody; FRCP2 antibody; Irisin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FNDC5 recombinant protein (Accession Number: NM_001171941). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse liver, mouse skeletal muscle and rat liver were subjected to SDS PAGE followed by western blot with Catalog No:110738(FNDC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours
Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:110738(FNDC5 Antibody) at dilution of 1:50
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Background
fibronectin type III domain containing 5（FNDC5）encodes 23kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. The exercise induces muscle FNDC5 expression. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.
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