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Product Name
FN3KRP antibody
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Description
FN3KRP Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, human kidney tissue. Positive IF detected in HeLa cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ12171 antibody; FN3K related protein antibody; FN3K RP antibody; FN3KL antibody; FN3KRP antibody; Ketosamine 3 kinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FN3KRP recombinant protein (Accession Number: NM_024619). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human brain using Catalog No:110735(FN3KRP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:110735(FN3KRP antibody) at dilution of 1:50 (under 40x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110735(FN3KRP Antibody) at dilution of 1:600
Immunofluorescent analysis of HeLa cells using Catalog No:110735(FN3KRP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Fn3KRP (Fn3K-related protein) are believed to be the first examples of deglycating enzymes acting on the Amadori product to reverse the early stages of the Maillard reaction in vivo in higher organisms (PMID: 17337043). Fn3KRP possesses an ATP-binding domain that is structurally related to that of both the aminoglycoside kinases and eukaryotic protein kinases. Fn3KRP possesses an intrinsic activity towards ketosamine substrates (PMID: 18637789). Catalog#16312-1-AP is a rabbit polyclonal antibody raised against the full-length of human FN3KRP. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.
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