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Product Name
Flotillin 1 antibody
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Description
Flotillin 1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse heart tissue, mouse brain tissue, rat heart tissue. Positive IF detected in Hela cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLOT1 antibody; flotillin 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Flotillin 1 recombinant protein (Accession Number: XM_005248780). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:110722(FLOT1 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using FLOT1 antibody Catalog No:110722 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-Flotillin 1 (IP:Catalog No:110722, 4ug; Detection:Catalog No:110722 1:500) with mouse brain tissue lysate 4400ug.
1X10^6 HeLa cells were stained with 0.05ug Flotillin 1 antibody (Catalog No:110722, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IgG with dilution 1:100.
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Background
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
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References
- Chen X, Li W, Ren J. Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death. Cell research. 24(1):105-21. 2014.
- Liu J, Huang W, Ren C. Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways. Scientific reports. 5:11614. 2015.
- Sohda M, Misumi Y, Oda K. TNFα triggers release of extracellular vesicles containing TNFR1 and TRADD, which can modulate TNFα responses of the parental cells. Archives of biochemistry and biophysics. 587:31-7. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"