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Product Name
FLCN antibody
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Description
FLCN Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, A549 cells, HeLa cells, mouse kidney tissue. Positive IP detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 38-45 kDa,64 kDa,20-26 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BHD antibody; DKFZp547A118 antibody; FLCL antibody; FLCN antibody; FLJ45004 antibody; FLJ99377 antibody; folliculin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FLCN recombinant protein (Accession Number: NM_144606). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:110686(FLCN antibody) at dilution of 1:300
IP result of anti-FLCN(Catalog No:110686) with Hela cell.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110686(FLCN antibody) at dilution of 1:50 (under 40x lens)
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Background
FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma. This antibody can recognize all the 3 isoforms of FLCN.
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References
- Lim TH, Fujikane R, Sano S. Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA. DNA repair. 11(3):259-66. 2012.
- Sano S, Sakagami R, Sekiguchi M, Hidaka M. Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O⁶-methylguanine-induced apoptosis. Biochemical and biophysical research communications. 430(2):810-5. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"