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Product Name
FGD5 antibody
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Description
FGD5 Rabbit Polyclonal antibody. Positive IHC detected in human lung. Positive IF detected in HepG2. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 160 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FGD5 antibody; ZFYVE23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FGD5 recombinant protein (Accession Number: BC035364). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110628(FGD5 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung using Catalog No:110628(FGD5 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:110628(FGD5 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using FGD5 antibody Catalog No:110628 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
FGD5 (or ZFYVE23) is an endothelial specific gene, plays a crucial role in vascular pruning. Deficient expression of FGD5 markedly inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model. FGD5-deficient endothelial cells fail to migrate. Moreover, the cells are susceptible to proapoptotic stimuli associated with VEGF-stimulated activation of Akt. FGD5 promotes apoptosis-induced vaso-obliteration via induction of the hey1-p53 pathway by direct binding and activation of CDC42.
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References
- Nakhaei-Nejad M, Haddad G, Zhang QX, Murray AG. Facio-genital dysplasia-5 regulates matrix adhesion and survival of human endothelial cells. Arteriosclerosis, thrombosis, and vascular biology. 32(11):2694-701. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"