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Product Name
Fetuin-A antibody
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Description
Fetuin-A Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, human liver tissue. Positive IP detected in HepG2 cells. Positive WB detected in Hepg2 cells, human blood tissue, human heart tissue. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
A2HS antibody; AHS antibody; AHSG antibody; alpha 2 HS glycoprotein antibody; Alpha 2 Z globulin antibody; Ba alpha 2 glycoprotein antibody; FETUA antibody; Fetuin A antibody; HSGA antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of Fetuin-A recombinant protein (Accession Number: NM_001622). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107206(AHSG antibody) at dilution of 1:5000
IP Result of anti-AHSG (IP:Catalog No:107206, 3ug; Detection:Catalog No:107206 1:3000) with HepG2 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:107206(AHSG antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:107206(AHSG antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using AHSG antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces insulin sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.
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