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Product Name
FEM1C antibody
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Description
FEM1C Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human heart tissue, mouse brain tissue. Positive WB detected in HepG2 cells, mouse brain tissue, mouse heart tissue. Observed molecular weight by Western-blot: 69 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
EUROIMAGE686608 antibody; EUROIMAGE783647 antibody; fem 1 homolog c (C. elegans) antibody; FEM1 gamma antibody; FEM1A antibody; FEM1C antibody; KIAA1785 antibody; Protein fem 1 homolog C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FEM1C recombinant protein (Accession Number: XM_005272035). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110617(FEM1C antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:110617(FEM1C Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:110617(FEM1C Antibody) at dilution of 1:50 (under 40x lens)
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Background
Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"